Description

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:

With Without

• GlutaMAX™ • HEPES

• Phenol Red  

The complete formulation is available.

Using DMEM/F-12

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2environment to maintain physiological pH.

cGMP manufacturing and quality system

DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12 product made in our Scotland facility (31331-028). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.

 

Specifications

Glutamine: GlutaMAX™-I

Phenol Red Indicator: Phenol Red

Form: Liquid

HEPES Buffer: No HEPES

Sodium Pyruvate Additive: Sodium Pyruvate

Serum Supplementation: Standard Serum Supplementation

Glucose: High Glucose

Product Line: Gibco®, GlutaMAX™

Product Size: 500 mL

Volume (Metric): 500 ml

Green Features: Sustainable packaging

Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C. Protect from light

Shipping conditions: Ambient

Shelf life: 12 months from date of manufacture